Metabolic Measurements of Embryos
Analysis of pyruvate utilization by least-square analysis revealed a significant (P>0.001) increase in 14CO2 production from embryo's stored in EHM (MOPS buffered EMCARE™ media) compared to OCM (Ovum Culture Media). Chi-square analysis of re-development to hatching also showed a significant (P>0.001) improvement in embryonic viability after storage in EHM at 25°C. Both 14CO2 production and development after storage significantly decreased from 24 to 48h.
Embryo toxicity during development was assessed using in vitro-produced cattle embryos. It was concluded that a 10mM concentration is less effective than 20mM, zwitteronic buffers are better without CO2 enrichment, and MOPS (buffer) was less toxic in terms of cleavage than either HEPES or BES (buffers). In fact, under MOPS, 20mM, no CO2, 87% of embryo's reached the cleavage stage, whereas only 73% of HEPES, 20mM, no CO2, reached cleavage. A difference of 19.2% in efficacy.
For more information refer to the EMCARE™ US Patent 5,716,847.